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. Author manuscript; available in PMC: 2010 Mar 2.
Published in final edited form as: Gastroenterology. 2005 Aug;129(2):682–695. doi: 10.1016/j.gastro.2005.05.050

Figure 2.

Figure 2

Immunohistochemical localization of endothelin-1 (ET)-1, cytokeratin 19 (CK19), and α-smooth muscle actin (α-SMA) in liver sections from CBDL animals. Representative images are shown of ET-1 (top), CK19 (middle), and α-SMA (bottom) in normal (A, E, and I), 1-week (B, F, and J), 2-week (C, G, and K), and 3-week (D, H, and L) CBDL liver. In normal liver (A), ET-1 staining was most prominent in a pattern consistent with sinusoidal endothelial staining. After CBDL (B–D), staining increased markedly in the cytoplasm of biliary epithelial cells and focally in periductal regions. CK19 stained the biliary epithelium in normal (E) and CBDL (F–H) animals in a pattern similar to CBDL ET-1 staining. In normal liver (I), α-SMA staining was most prominent in vascular smooth muscle. After CBDL (J–L), α-SMA staining increased in the periductal regions (but not in biliary epithelium) in a pattern consistent with stellate cells or portal myofibroblast staining (original magnification, 40×).