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. 2010 Mar;176(3):1323–1338. doi: 10.2353/ajpath.2010.090503

Figure 3.

Figure 3

B. abortus infects and multiplies in astrocytes and microglia. Infection with B. abortus was performed at different multiplicity of infection (MOI), and CFU was determined after 24 hours postinfection in astrocytes (A) and microglia (B) cultures. Astrocytes, microglia, and J774.A1 cells were infected with B. abortus (MOI 100), and replication within each cell type was assessed by determination of CFU after 2, 8, 16, 24, or 48 hours (C). ELISA determination of proinflammatory cytokines (IL-6, IL-1β, and TNF-α) and chemokines (KC and MCP-1) in culture supernatants of 24-hour infected astrocytes (D) and microglia (E). Bars and symbols express the mean ± SEM of duplicates. Data shown are from a representative experiment of three performed. *P < 0.05; **P < 0.01; ***P < 0.001 versus N.I. (not infected).