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. 2009 Dec 24;19(6):1058–1065. doi: 10.1093/hmg/ddp569

Figure 2.

Figure 2.

PABPN1 is required for normal myoblast proliferation. Pure cultures of primary mouse myoblasts from limb, pharyngeal and extraocular (EOM) muscles were transfected with one of two siRNA oligonucleotides for PABPN1 [PABPN1-1 (1) or PABPN1-2 (2)] or a scrambled control (C). Cells were labeled with BrdU and immunostained with anti-BrdU antibody. (A) Protein extracts were analyzed by immunoblotting for PABPN1 to confirm knockdown. Alpha-tubulin was used as a loading control. (B) Representative BrdU immunostaining after siRNA transfection (bar, 100 µm). DAPI staining was used for nuclear visualization. (C) The relative percentage of BrdU+ nuclei was determined in limb (L), pharyngeal (Ph) and extraocular (EOM) myoblasts and fibroblasts (F) transfected with siRNA. PABPN1 knockdown results in ∼40% decrease in the percentage of BrdU+ cells. Data are mean ± SE, *P < 0.05, n = 3.