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A, representative MS/MS spectra of the tryptic peptides derived from the pulled down proteins (TIF1β, AN32A, and BZW1) from MCF-7 cells. Enhanced a1 ions were detected in all spectra. B, the enrichment factor (ratio values) obtained from stable isotope dimethyl labeling (open) and from Western blotting (WB) (black). No significant differences between the two methods were found for all three proteins (n = 3, p > 0.05).
