Table I. Summary of SP-PLA performance characteristics for detection of VEGF, IL-8, and IL-6.
VEGF |
IL-8 |
IL-6 |
|||||||
---|---|---|---|---|---|---|---|---|---|
Buffer | Serum | Plasma | Buffer | Serum | Plasma | Buffer | Serum | Plasma | |
LOD (fm) | 1.9 | 10.7 | 5.2 | 2.8 | 20 | 15.6 | 8.9 | 20 | 11 |
Linear range | 106 | 105 | 105 | 106 | 106 | 106 | 106 | 106 | 106 |
Intra-assay variation (CV%) | 21.3 | 7.8 | 10.6 | 24.8 | 16.1 | 21 | 16 | 7.5 | 13.8 |
R2 | 0.998 | 0.986 | 0.996 | 0.988 | 0.990 | 0.989 | 0.999 | 0.984 | 0.997 |
Recovery (%) | 105.6 | 100.1 | 94.8 | 99.4 | 107.6 | 107.1 | 100.7 | 113 | 102.4 |