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Fraction composition based on proteomic markers (A and B). The percentage of different organelles in each fraction was calculated from the distribution of the organelle-specific marker ensembles among the fractions (see “Results” for details). Nuc, nuclei; Mito, mitochondria; PM, plasma membrane; D.Mic, dense microsomes; Cyto, cytosol; KLA, Kdo2-lipid A-activated cells. The data are mean ± S.E. for three independent preparations.
