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Distribution of selected protein markers among pure organelles (A–D). Protein content in pure organelles was calculated based on the organellar composition of fractions (Fig. 4) and protein distribution in fractions (measured in the iTRAQ experiment). See text for detail on calculations. Nuc, nuclei; Mito, mitochondria; PM, plasma membrane; D.Mic, dense microsomes; Cyto, cytosol; KLA, Kdo2-lipid A-activated cells. The data are mean ± S.E. for three independent preparations.
