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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Anal Biochem. 2009 Oct 21;398(1):15–23. doi: 10.1016/j.ab.2009.10.031

Fig. 1.

Fig. 1

Excitation and emission spectra of (A) Eu(III)-DTPA-PEG9-NDP-α-MSH and (B) Eu(III)-DOTA-PEG9-NDP-α-MSH (each 10 nM) using DELFIA. The compounds were excited at 340 nm. Acidification: Samples (20 μL) were incubated with 2.0 M HCl (50 μL) for 5 hours, neutralized with 2.0 M NaOH (55 μL), treated with the enhancement solution (115 μL), and incubated for 30 min before reading.