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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nat Protoc. 2009 Dec 17;5(1):51–66. doi: 10.1038/nprot.2009.197

Figure 2. MnSOD and CuZnSOD activity gels.

Figure 2

To determine a change in activity of the antioxidant proteins CuZnSOD and MnSOD in MCF-10A immortalized breast epithelial cells after infection with the AdEmpty, AdCuZnSOD or AdMnSOD (50 or 100 multiplicity of infectivity, MOI) adenoviral vector constructs, an activity gel was performed. Protein (100 μg) was loaded and the gels were electrophoresed at 4 °C. SOD expression was visualized by first soaking gels in NBT. MnSOD expression alone was visualized by the addition of NaCN which inhibits CuZnSOD activity. The adenoviral vector AdCuZnSOD increased CuZnSOD activity while the AdMnSOD vector increased MnSOD activity.