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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nat Protoc. 2009 Dec 17;5(1):51–66. doi: 10.1038/nprot.2009.197
Reagent Volume for an 8% Gel Volume for a 12% Gel
ddH2O 10.95 ml 8.48 ml
30% Acyl-Bis (wt/vol) 4.8 ml 7.28 ml
Tris separating buffer, pH 8.8 2.25 ml 2.25 ml
TEMED 9 μl 9 μl
10% APS (wt/vol) 68 μl 68 μl