| Reagent | Volume required |
| 30% Acyl-Bis (wt/vol) | 1.0 ml |
| Tris Stacking Buffer, pH 6.8 | 1.6 ml |
| 40% Sucrose (wt/vol) | 3.2 ml |
| 0.004% Riboflavin-5’-phosphate (wt/vol) | 800 μl |
| TEMED | 4 μl |
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