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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nat Protoc. 2009 Dec 17;5(1):51–66. doi: 10.1038/nprot.2009.197
Solutions Final Concentrations Volume required for SOD System
PB with DETAPAC and BSA 0.05 M PB, 1 mM DETAPAC, 0.13 mg BSA 12.9 ml
Catalase 40 U ml-1 1 U 0.5 ml
Xanthine 1.18 mM 100 μM 1.7 ml
NBT 2.24 56 μM 0.5 ml
PB 0.05 M 0.3 ml
BCS 10 mM 50 μM 0.1 ml
Total volume 16.0 ml