Solutions | Final Concentrations | Volume required for SOD System |
PB with DETAPAC and BSA | 0.05 M PB, 1 mM DETAPAC, 0.13 mg BSA | 12.9 ml |
Catalase 40 U ml-1 | 1 U | 0.5 ml |
Xanthine 1.18 mM | 100 μM | 1.7 ml |
NBT 2.24 | 56 μM | 0.5 ml |
PB | 0.05 M | 0.3 ml |
BCS 10 mM | 50 μM | 0.1 ml |
Total volume | 16.0 ml |