|
→ | initial absorbance | |
→ | absorbance at 60 s | ||
|
→ | total mls in reaction; both blank and sample | |
→ | total mls of sample; both blank and sample (example if 20 μl of sample was added before splitting the sample in half, denominator = 0.02 ml) | ||
|
→ | divide by the protein concentration of the original sample |