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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nat Protoc. 2009 Dec 17;5(1):51–66. doi: 10.1038/nprot.2009.197
k=160lnA0A60
initial absorbance
absorbance at 60 s
ktotal/ml=k×6ml sample
total mls in reaction; both blank and sample
total mls of sample; both blank and sample (example if 20 μl of sample was added before splitting the sample in half, denominator = 0.02 ml)
k/mg=ktotal/mlmgml­1
divide by the protein concentration of the original sample