Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Mar 1;5(3):e9463. doi: 10.1371/journal.pone.0009463

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Hauser et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Shamporter is a modified anti podocyte antibody that piggybacks siRNA to a target cell. (A) Specific cleaving of the anti mouse podocyte divalent IgG at the inter-heavy chain disulfide bond, using 2-Mercaptoethylamine, results in monovalent IgG. (B) A Neutravidin binding site is conjugated to the available sulfohydryl group. (C) Protamine is biotinylated which then binds to the monovalent IgG. (D and E) Negatively charged siRNA molecules bind to the positively charged protamine domain of the shamporter construct. (F) The modified antibody (shamporter) carrying siRNA binds to the podocytes. The bound antibody is internalized and transported to the cytoplasm. Uncoating of the endosomatic vesicle releases the transported siRNA into the cytoplasm. The released siRNA activates RISC (RNA induced silencing complex), which is followed by degradation of the target mRNA.