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. 2010 Mar 1;5(3):e9463. doi: 10.1371/journal.pone.0009463

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Hauser et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The antibody binds to the apical, basal and the slit diaphragm regions of the podocyte (A). A small number of immunogold particles were present in the glomerular basement membrane (GBM) and endothelial cells (GEN). The arrows indicate examples of immunogold particles. Sheep anti mouse podocyte antibody staining was detected only in the glomerulus (B + D arrows) and was absent in the tubular compartment (D asterix). Control animals injected with normal sheep IgG showed no staining (C + E).