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. 2010 Mar 1;5(3):e9463. doi: 10.1371/journal.pone.0009463

Figure 4. Western blot analyses for p57, CDK5 and TRPC6.

Figure 4

In cultured immortalized mouse podocytes transfected with shamporter + siRNA directed against p57 (A) or CDK5 (B), there was a progressive decrease in protein levels at 48 h and 72 hours. In cultured podocytes transfected with shamporter + siRNA directed against TRPC6, there was a dose dependent decrease in protein levels for TRPC6 (C). Cells transfected with nonfunctional control-siRNA served as control. The panels on the right show densitometry performed against loading controls GAPDH or β-Actin.