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. 2010 Mar 1;5(3):e9463. doi: 10.1371/journal.pone.0009463

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Hauser et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Animals injected with shamporter + control siRNA show a strong linear staining signal for nephrin and podocin, as well as glomerular staining for TRPC6. In animals injected with shamporter + nephrin or TRPC6 specific siRNA, the staining signal was significantly reduced, while the signal for podocin remained unchanged. When nephrin, podocin, or TRPC6 antibodies were omitted, no staining was detected.