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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: Vaccine. 2010 Feb 23;28(8):2011–2019. doi: 10.1016/j.vaccine.2009.10.059

Fig 2.

Fig 2

Analyses V protein expression by the wild type (wt.V) or codon optimized (V.opt) LcrV DNA vaccine. A. Western-blot analysis of V protein expression by V.opt or wt.V DNA vaccines, or empty vector in supernatant (S) and lysate (L) of transfected 293T cells. B. V protein amount was determined by a quantitative ELISA in 293T cell lysate transfected with either V.opt or wt.V DNA vaccines. The V protein concentration in 293T cell lysate was calculated based on a standard curve of a control recombinant V protein. V-specific mouse sera were used as the detection antibody for Western blot (1:500 dilution) or ELISA (1:1000 dilution).