Table 1.
Molecular cytogenetic techniques, their resolution and validity for single-cell analysis of interphase/metaphase chromosomes (for more details see text)
| Approach | Resolution | MA* | IA** | SCA^ | PVC^^ | Refs |
|---|---|---|---|---|---|---|
| Cytogenetic banding analysis ("golden standard") | 5-7 Mb | + | - | + | + | [1] |
| FISH/MFISH/SKY | ||||||
| FISH/MFISH/SKY with centromeric probes | >0.3-1 Mb | +/- | + | + | +/- | [20-26,30,32,35,41,42,44,46] |
| FISH/MFISH/SKY with site-specific probes | ~0.1-2 Mb | +/- | +/- | +/- | +/- | [45,47-50] |
| FISH/MFISH/SKY with whole-painting probes | >5-10 Mb | + | - | + | + | [2,3,5,6,13,36,39,40] |
| MCB | ||||||
| Metaphase MCB | ~2-5 Mb | + | - | + | + | [2,13,43,45] |
| ICS-MCB | ~2-5 Mb | - | + | + | + | [23,24,26,28,29,31-35] |
| Fiber FISH | >2.3 (2-3) kb | na | na | + | + | [51,52] |
| Single-cell CGH | ||||||
| Standard CGH | 2-5 Mb | na | na | + | - | [53] |
| Array CGH | 0.03-1 Mb | na | na | + | - | [14,54] |
* -- analysis of metaphase chromosomes (MA - metaphase analysis); ** -- analysis of interphase chromosomes (IA - interphase analysis); ^ -- possibility to perform single cell analysis (SCA); ^^ -- possibility to visualize chromosomes or chromosomal loci (PVC - possibility to visualize chromosomal loci); na - not applicable;