Table 2.

Overview of I-FISH techniques

Technique	Brief description	Advantages	Limitations	Refs
I-FISH with centromeric probes	I-FISH on interpahse nuclei painting pericentromeric (heterochromatic) regions	High hybridization efficiency, chromosome specifity (apart few chromosomes)	Signal associations, impossible to analyze chromosomes 5,13, 14, 19, 21, 22; heteromorphisms	[7,10,20-22,30,35,41,58-60,81,82]
I-FISH with site-specific probes	I-FISH painting specific euchromatic genomic loci	Small specific genomic loci are visualized	Low hybridization efficiency, numerous artifacts	[8,13,28,42,47-50,69]
I-FISH with wcp	I-FISH painting chromosome territories	Identification of nuclear chromosome territories	Chromosome territories are ambiguous, no additional information	[57,70-72,93]
mFISH	Multicolor I-FISH with >2 probes labeled by different fluorochromes/ligands	Analysis of several targeted genomic loci	Difficulty to distinguish between artifacts and aneuploidy/polyploidy	[7,10,20-22,30,35]
mFISH/QFISH	mFISH + QFISH digitalization of FISH signals	Distinguishes between FISH artifacts and aneuploidy (polyploidy)	Same as mFISH	[7,10,24-29,32,35]
MFISH	Simultaneous visualization of the complete set of chromosomes in an interphase nucleus	All chromosome territories are simultaneously seen	Exceedingly sophisticated analysis; data poorly interpretable	[70,93]
ICS-MCB	Chromosome-specific MCB generated on interphase nuclei	Visualization of whole banded interphase chromosomes in their integrity	A pair of homologous chromosomes is studied per assay; relative complexity of the analysis	[7,10,13,19,23,24,26-29,31,33-35,74,75]