Figure 1.

Female gonadal re-aggregation prior to ovary maturation impaired oocyte development. (A) Female mouse genital ridges and ovaries from e11.5 (embryonic day) to P2 (post-natal day) were disrupted by re-aggregation, transplanted under the kidney capsule for three weeks, and sections were examined by H&E stain for oocyte development in follicles in comparison to intact (B) gonadal transplant controls. (C) For comparison, in vivo control endogenous time points are shown to illustrate e12.5 genital ridge, pre-follicle formation e14.5 differentiating ovary, and post-follicle formation adult ovary. (D) P2 ovaries were also FACS sorted prior to re-aggregation to ensure a single cell suspension and an equivalent dissociation method. 100× magnification.