Figure 2.

Quantification of re-aggregation induced impairment of oocyte development. (A) The average number of oocytes in follicles per graft, normalized to the number of sections counted, was determined following three weeks transplantation of intact or re-aggregated samples. No oocytes were detected in re-aggregated samples prior to e13.5. Oocyte numbers increased in re-aggregated samples after e13.5 in parallel to the increasing age of ovary donors, however, oocyte numbers were still significantly reduced from e13.5 to e15.5 compared to intact control samples. (B) Average weight per graft following transplantation of re-aggregated early and late gonadal stages demonstrating equivalent weights. Error bars represent s.d. (n = 3, 150 sections/graft). * = p < 0.05 between e16.5 and e15.5 re-aggregated. **/*** = p < 0.05 between e12.5/e14.5 intact and re-aggregated, respectively.