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Representative Western blot and quantitative analysis of signal density on blots from three independent experiments analyzing GaLV TM levels in lysates of 293T cells co-transfected with GaLV Env, pHR' and the indicated packaging plasmids. (SN) indicates Vpu containing substitutions S52N and S56N. Immunological detection of HIV-1 p24 and cellular actin levels were included as controls.
