Table 1.
Replication capacity of HIV-1 with mutations in the p51↓RNH cleavage site ± T477A
| RT p51↓RNH mutation | Virus titer (% wild-type control)a | ||
|---|---|---|---|
| - T477A | + T477A | ||
| WT | 100 | 100 ± 30 | (N.S.)b |
| F440V | < 1 ± 1 | 10 ± 3 | (p < 0.01) |
| T439S/V442G | ---c | --- | |
| Y441I/V442K | --- | --- | |
| F440A | <1 ± 1 | 10 ± 5 | (p < 0.01) |
| F440A/Y441A | <1 ± 1 | 1 ± 1 | (N.S.) |
| F440W/Y441W | <1 ± 1 | 32 ± 14 | (p < 0.01) |
| E438N | <1 ± 1 | 5 ± 4 | (p < 0.5) |
a Infectious virus titer (TCID50/ml) was determined in MT-2 cells and was normalized by dividing by the amount of viral p24 (ng/ml), as described in Materials and Methods. Data are presented as mean % wild-type virus titer ± standard deviation from four individual experiments.
b P values were calculated using a one-tailed Student's t-test assuming equal variance. N.S., not significant.
c No virus titer noted