Figure 2.

Infection and injury increase κB-luc reporter activity in living flies. (A) The plasmid used for generation of κB-luc flies. Plasmid contained 8 copies of κB binding sequences, a heat shock protein (Hsp) 70 Bb basal promoter and a luciferase open reading frame (ORF). (B) Mean ± SEM luciferase activity (arbitrary units) was measured at indicated ZT times when κB-luc flies were treated at ZT 18. **p < 0.0001 and *p < 0.01, student's t-test compared to HC at corresponding time points. n = 32 flies for each group.