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. 2010 Mar 3;5(3):e9491. doi: 10.1371/journal.pone.0009491

Figure 2. Characterization of T cell hybridoma clone 15HEL.

Figure 2

MN (5×104/well) were incubated with antigen (intact HEL for A and C, HEL peptides for B) and 15HEL (1×105/well) for 24 hrs. Supernatants were harvested, and IL-2 measured by ELISA. A. Effect of anti-HLA-DR or isotype on response. B. Identification of HEL peptide epitope recognized by 15HEL. C. Effect of anti-CD80 and CD86 antibodies together (5 µg/ml of each) or isotype control (10 µg/ml) on responses. Results of A and C represent triplicate wells and error bars SEM.