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. 2010 Mar 3;5(3):e9511. doi: 10.1371/journal.pone.0009511

Table 2. Theorem representing the reactions of the glycolytic pathway leading from D-Glucose to Fructose-1,6-bisphosphate: Glc&HK&GPI&PFK&ATP&ATP ⊢ F1,6P.

1. Glc & HK & GPI & PFK & ATP & ATP IA
2. Glc & HK From 1 by &E
3. GPI From 1 by &E
4. PFK From 1 by &E
5. ATP From 1 by &E
6. ATP From 1 by &E
7. Glc & HK → Glc*HK EVF
8. Glc*HK From 2,7 by →E
9. (Glc*HK) & ATP From 5,8 by &I
10. (Glc*HK) & ATP → (Glc*HK) *ATP EVF
11. (Glc*HK) *ATP From 9,10 by →E
12. (Glc*HK) *ATP → G6P & HK & ADP EVF
13. G6P & HK & ADP From 11,12 by →E
14. G6P From 13 by &E
15. HK From 13 by &E
16. ADP From 13 by &E
17. G6P & GPI From 3,14 by &I
18. G6P & GPI → G6P*GPI EVF
19. G6P*GPI From 17,18 by →E
20. G6P*GPI → F6P & GPI EVF
21. F6P & GPI From 19,20 by →E
22. F6P From 21 by &E
23. GPI From 21 by &E
24. F6P & PFK From 4,22 by &I
25. F6P & PFK → F6P*PFK EVF
26. F6P*PFK From 24,25 by →E
27. (F6P*PFK) & ATP From 6,26 by &I
28. (F6P*PFK) & ATP → (F6P*PFK) *ATP EVF
29. (F6P*PFK) *ATP From 27,28 by →E
30. (F6P*PFK) *ATP → F1,6P & PFK & ADP EVF
31. F1,6P & PFK & ADP From 29,30 by →E
32. F1,6P From 31 by &E
33. (Glc & HK & GPI & PFK & ATP & ATP) → F1,6P From 1–32 by →I

The reactions of the pathway are illustrated in all their detail. In each line we write the conclusion of a rule application, together with its justification, without keeping track of the initial aggregates (IA) on which it may depend. All the lines in this example depend on the IA of line 1, except for the EVFs, which do not depend on any IA, and the final theorem reported on line 33. Here, the IA of line 1 is “discharged” by the application of rule →I, as indicated to the right of line 33 (From 1–32 by →I), with the consequence that the final theorem does not depend on any IA. Abbreviations: D-Glucose, Glc; D-Glucose-6-phosphate, G6PPP; Hexokinase [EC 2.7.1.1], HK; Glucose-6-phosphate isomerase [EC 5.3.1.9], GPI; 6-Phosphofructokinase [EC 2.7.1.11], PFK; Fructose-6-phosphate, F6PP; Fructose-1,6-bisphosphate, F1,6PP. Note that no formula is used more than once in the derivation process.