Figure 5. Analyses of the gadX-uspE interaction through knockout studies.

GFP fluorescence of wild type E. coli BW25113 and single gene knockout mutant ΔgadX carrying the reporter plasmid puspE-gfp, and wild type E. coli and single gene knockout mutant ΔuspE carrying the other reporter plasmid pgadX-gfp. Cells of each reporter strain were cultured in LB broth at 30°C overnight and re-suspended in 1×PBS before cell density (OD) and fluorescence intensity were measured. GFP fluorescence for each strain was normalized to the OD value. Error bars indicated standard deviations from two replicated cultures each with four replicate readings.