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. 2010 Feb 27;46(2):157–167. doi: 10.3164/jcbn.09-97

Fig. 6.

Fig. 6

PQ treatment impaired insulin-induced phosphorylation of mTOR. (A) phosphorylation of mTOR was analyzed with primary cultured rat hepatocytes pre-treated with/without 2 mM PQ for 6 h and then treated with/without 100 nM insulin for 10 min. Protein lysate was analyzed by Western blot analysis with anti-phospho-mTOR (Ser 2448) antibody. Phosphorylated levels were quantified and expressed as relative values to cells without insulin and PQ treatment (Ins-, PQ 0 mM). Values are means ± SEM of three dishes. #Significantly different from the cells without insulin treatment (p<0.05). (B) The effect of rapamycin treatment on the IGFBP-1 mRNA level was measured by RT-PCR with primary cultured rat hepatocytes treated with/without 10 nM rapamycin and 1 µM insulin for 6 h simultaneously. Values represent the repressive effect of insulin on the IGFBP-1 mRNA level (1 µM insulin/0 M insulin) and are expressed as means ± SEM of three dishes. *Significantly different between the two groups by rapamycin treatment (p<0.05).