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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cancer Res. 2010 Feb 16;70(5):1960–1969. doi: 10.1158/0008-5472.CAN-09-3535

Figure 4.

Figure 4

Obatoclax activation of Bax takes place through a direct mechanism, which is attenutated by site directed mutagenesis of the lysine 21 residue. Wild-type (A) recombinant human Bax or K21E mutant Bax was incubated with increasing doses of obatoclax. An increasing amount of active Bax was immunoprecipitated with obatoclax treatment in wild-type Bax, which was attenuated in the K21E mutant protein. Cross-linking of recombinant wild-type Bax protein (B) demonstrated increasing multimers with obatoclax treatment, which were not present in mutant protein. Normalized densitometric ratios are provided beneath the figure.