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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Gastroenterology. 2009 Nov 18;138(3):1046–1054. doi: 10.1053/j.gastro.2009.11.043

Figure 6. Treg depletion in HP-DC transferred mice enhances H pylori–specific Th17 response and is correlated with lower level of bacterial colonization.

Figure 6

C57BL/6 mice (n = 10 per group) were injected IP with PBS or PC61 (anti-CD25 monoclonal antibody, 1 mg per mouse) on day 0. Mice were then immunized with HP-DC (106 cells per injection) on days 0 and 14. Mice were orally challenged with H pylori SS1 (108 organisms) three times in 1 wk starting on day 21. Mice were sacrificed on day 35 (2 wk postinfection) or given another IP injection of PC61 on day 30 and then sacrificed on day 65 (6 wk postinfection). (A) Percentages of CD4+CD25+ Tregs from splenocytes were determined by FACS. Treatment with PC61 reduced the percentage of Tregs in mouse spleen. (B) CD4+ T cells isolated from mouse spleens using MACS MicroBeads were stimulated with PBS or H pylori sonicate in the presence of unstimulated DCs. IL-17 concentrations were measured by ELISA. PC61 treatment significantly increased the production of H pylori–specific IL-17 by CD4+ T cells (n = 10 mice per group, * P <.05). (C-D) Gastric Foxp3 and IL-17 mRNA expressions are shown. (E) H pylori colonization was lower in PC61-treated mice. (F) Gastritis score was determined in a blinded fashion by Dr. Eaton.44