Cell proliferation (BrdU), macpiwi (PIWI), macboule (BOULE) and macactin (ACTIN) expression dynamics at 3 weeks following irradiation with doses of 30–90 Gy (protocol P1). Anterior is to the top (e eyes). a
1–6 Somatic stem cell proliferation recovered at all radiation doses. b
1–6
Macpiwi expression was completely restored in the gonads (t testes, o ovaries, de developing eggs) and in the somatic stem cell population (arrowheads; b
1–5), except at the highest radiation dose (b
6). c
1–6
Macboule expression was reconstituted at all radiation doses. d
1–6 Expression of the housekeeping gene macactin was identical to that in control animals. Note the smaller body size of animals irradiated with higher doses (a
6, b
6, c
6, d
6). Bar 100 µm (all worms)