Effect of irradiation on the regeneration process (red dotted lines level of amputation, red dotted circles regeneration blastema). In all images, anterior is to the left. a-d’ Non-irradiated regenerates clearly formed a regeneration blastema after 1–2 days (a-b’). c, c’ A forming stylet (st, open arrowheads) could be observed within the 3-day-old blastema. d, d′ After 5 days, the stylet (st) and seminal vesicle (sv) were rebuilt. e-g’ In irradiated regenerates exposed to a sublethal dose of 60 Gy, blastema formation was delayed. h, h’ Despite this delay, the lost body part was rebuilt within 21 days, when a full stylet and a seminal vesicle were observed. i-l’ In regenerates exposed to a lethal dose of 210 Gy, no regeneration blastema could be detected. l, l’ By 1 week after being cut, animals gradually started to disintegrate. Bars 100 µm (a-l), 40 µm (a’-l’)