Fig. 7.
Radiation of hatchlings with 60 Gy (a 1 -h 2) or 210 Gy (i-l). Note the recovery of the proliferating somatic stem cell population at 1 h (a 1 -b 2), 1 day (c 1 -d 2), 7 days, (e 1 -f 2) and 14 days (g 1 -h 2) post-irradiation (green BrdU labelling for S-phase cells, red anti-Phos-H3 staining for mitoses). At 14 days, control animals possessed testes (t in g 1, g 2), whereas irradiated worms had not yet developed gonads, although they had normal body length (h 1, h 2). i–l Morphological effect of lethal 210 Gy radiation on hatchlings; 18-day-old control animals (i, j)developed testes (t), ovaries (o), stylet (st) and a seminal vesicle filled with sperm (sv), whereas irradiated hatchlings (k, l) showed tissue disorganization. Note the absence of developing testes and ovaries. l Detail of the tail plate lacking a seminal vesicle or stylet. Bars 100 µm (a 1 -h 2, i, k), 40 µm (j, l)