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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Nat Chem Biol. 2010 Jan 31;6(3):209–217. doi: 10.1038/nchembio.304

Figure 3. Effects of LTM and cycloheximide on translation elongation in vitro and in vivo.

Figure 3

a–c. Polysome profiles of compounds in 293T cells. Cells were treated with each compound at the indicated concentrations before lysis and cell lysates were subjected to centrifugation through a 15–45% sucrose gradient. d. Polysome profiles in vitro. Capped [32P]-labeled rabbit β-globin RNA was incubated in rabbit reticulocyte lysate and indicated compound for 15 min before centrifugation through a 10-35% sucrose gradient. e. LTM prevents the ribosome from leaving the start codon. Toeprints of 2 mM Cycloheximide and 200 µM LTM compared to 1 mM GDPNP on rabbit β-globin mRNA (see METHODS SUMMARY for details). Each experiment was repeated at least once to ensure reproducibility.