Figure 1. Alveolar macrophage isolation and distribution.

Pathogen-free guinea pigs were randomly assigned to drinking water, ethanol in the drinking water (4%) or, ethanol + SAM in the drinking water. During the 25 days of treatment, the only access to drinking water was the experimental mixture. Solid diet was provided ad libitum to all three groups. After the appropriate period of experimental diet, the animals were lavaged and the cell pellet subfractionated on a Percoll gradient. A hemacytometer was used to count the percentage of cells obtained from each layer (A). Bars represent means ± SE. ^a p≤ 0.05 compared to control, ^b p≤ 0.05 compared to control+SAM, ^cp≤ 0.05 compared to EtOH+SAM ¹ p≤ 0.05 compared with layer 1 (within that group) and ² p ≤ 0.05 compared with layer 2 (within that group), ³ p ≤ 0.05 compared with layer 3 (within that group). n=5 or more guinea pigs in each group.