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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Alcohol Clin Exp Res. 2009 Jul 23;33(10):1782–1793. doi: 10.1111/j.1530-0277.2009.01017.x

Figure 3. Quantification of PU.1 in alveolar macrophage subpopulations.

Figure 3

Representative photomicrographs at 60X magnification are shown in Figure A. PU.1 expressions were determined by staining alveolar macrophages with primary antibodies directed against PU.1 and then secondary antibodies directed against IgG labeled with FITC. The RFU/field was determined by computer analysis of the confocal microscopic images (B). Bar height represents RFU/field ± SE and was tallied from at least 20 experimental fields. Bars represent means ± SE. a p≤ 0.05 compared to control, b p ≤ 0.05 compared to control +SAM, and cp≤ 0.05 compared to EtOH + SAM. n=5 or more fields per group.