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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Mol Microbiol. 2009 Nov 2;74(6):1331–1343. doi: 10.1111/j.1365-2958.2009.06945.x

Fig. 5.

Fig. 5

BosR controls the expression of OspC and DbpA in Bb. Bb was inoculated into BSK-H medium (pH6.8) at 1000 spirochetes/ml and grown at 37°C. Spirochetes were collected at late-log phase. A volume of whole-cell lysates equivalent to 4 × 107 cells was loaded per gel lane and Bb gene expression was assessed by SDS-PAGE (A) and immunoblot (B) analyses. Approximate molecular masses are indicated at the left in kDa. The arrow in (A) indicates the position of OspC in SDS-PAGE. Specific antibodies, indicated as α-, used in the immunoblot (B) are indicated on the right. Lane 1, WT B31; lane 2, bosR- OY10/D12; lane 3, mutant OY10/H3; lane 4, bosR-/+ OY34/E6; lane 5, bosR-/+ OY34/C4.