Table 3.
Validation of microarray results using quantitative RT-PCR
| Gene | Function | WT/bosR mutant | |
|---|---|---|---|
| microarray | qRT-PCR | ||
| bbb19 | outer surface protein C (ospC) | 87.76 | 38.96 |
| bbg27 | conserved hypothetical protein | 35.84 | 42.86 |
| bba24 | decorin binding protein A (dbpA) | 14.54 | 42.86 |
| bbo39 | ErpL protein (erpL) | 7.04 | 9.38 |
| bba48 | hypothetical protein | 5.14 | 1.25 |
| bbe31 | antigen, P35, putative | 4.37 | 4.05 |
| bbj26 | ABC transporter, ATP-binding protein | 4.32 | 4.11 |
| bb0565 | purine-binding chemotaxis protein (cheW-2) | 3.46 | 4.29 |
| bbf01 | ErpD protein, putative | 3.02 | 8.82 |
| bbr41 | conserved hypothetical protein | 2.87 | 2.24 |
| bb0071 | RNA polymerase sigma factor (rpoS) | 2.81 | 3.66 |
| bb0690 | neutrophil activating protein (napA) | a-2.11 | -1.57 |
| bb0763 | response regulatory protein (rrp-2) | -2.16 | -1.32 |
| bb0104 | periplasmic serine protease DO (htrA) | -2.18 | -1.49 |
| bb0153 | superoxide dismutase (sodA) | -2.25 | -1.37 |
| bba62 | lipoprotein | -2.33 | -2.07 |
| bb0032 | hypothetical protein | -2.53 | -2.04 |
| bb0401 | glutamate transporter, putative | -2.72 | -1.15 |
| bb0646 | hydrolase, alpha/beta fold family | -3.03 | -2.29 |
a
indicates the fold change of genes down-regulated in WT B31 relative to the bosR mutant.