Table 1.
Cluster | Sequence | Strand |
---|---|---|
U/8-oxoG+1+5 | 5′ CTCTTAGTCAGGAATAAXTATXAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATYCATACTTACGACCCTCGCGTCCG | 2 | |
U/8-oxoG+2−2 | 5′ CTCTTAGTCAGGAATAAXTATXAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCAYACTTACGACCCTCGCGTCCG | 2 | |
U/8-oxoG−1−5 | 5′ CTCTTAGTCAGGAATAAXTATXAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCATACYTACGACCCTCGCGTCCG | 2 | |
U/U+1 8-oxoG+5 | 5′ CTCTTAGTCAGGAATAAYTATXAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATYCATACTTACGACCCTCGCGTCCG | 2 | |
U/U+2 8-oxoG−2 | 5′ CTCTTAGTCAGGAATAAXTATYAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCAYACTTACGACCCTCGCGTCCG | 2 | |
U/U+5 8-oxoG+1 | 5′ CTCTTAGTCAGGAATAAXTATYAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATYCATACTTACGACCCTCGCGTCCG | 2 | |
U/U-1 8-oxoG−5 | 5′ CTCTTAGTCAGGAATAAXTATYAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCATACYTACGACCCTCGCGTCCG | 2 | |
U/U-2 8-oxoG+2 | 5′ CTCTTAGTCAGGAATAAYTATXAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCAYACTTACGACCCTCGCGTCCG | 2 | |
U/U-5 8-oxoG−1 | 5′ CTCTTAGTCAGGAATAAYTATXAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCATACYTACGACCCTCGCGTCCG | 2 | |
Tandem 8-oxoG | 5′ CTCTTAGTCAGGAATAAXTATXAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCATACTTACGACCCTCGCGTCCG | 2 | |
U control | 5′ CTCTTAGTCAGGAATAAGTATGAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCAYACTTACGACCCTCGCGTCCG | 2 | |
No lesion control | 5′ CTCTTAGTCAGGAATAAGTATGAATGCTGGGAGCGCAGGC | 1 |
3′ GAGAATCAGTCCTTATTCATACTTACGACCCTCGCGTCCG | 2 |
Y represents a uracil residue and X represents an 8-oxoG lesion. The lesions on strand 1 have been given a number relating their positions to the uracil residue on the complementary strand 2. The number denotes the base separation. A positive number is given if the lesion on strand 1 is in the 5′ direction to that on strand 2 and a negative number is given if the lesion on strand 1 is in the 3′ direction to that on strand 2. The U control oligonucleotide consists of a single uracil residue on strand 2.