Figure 7.
FRET emission scan showing absorption peak generated by incident light at 500 nm. Blue trace, wild-type CT fused to eYFP, 1 h post-induction, not subjected to nucleic acid digestion. Purple trace, wild-type CT fused to eYFP, pre-induction, not subjected to nucleic acid digestion. Red trace, wild-type CT fused to eYFP, 1 h post-induction, treated with DNase. Green trace, wild-type CT fused to eYFP, 1 h post-induction, treated with RNase. Orange trace, quadruple variant CT fused to eYFP, 1 h post-induction, not subjected to nucleic acid digestion.