Figure 8.
DNA labeling by end filling of the 11-nt 3′ recessive end. The 11-nt 3′ recessive end was generated by FokI/Nt.BtsCI. Strong fluorescent signal detected at the smaller DNA fragment (lower band) digested by FokI/Nt.BtsCI. The expected cleavage products and the 3′ recessive ends that can be labeled by end filling are illustrated at the lower panel. Green blocks, fluorescent labeled region; grey arrows, BtsCI top-strand cleavage site; white arrows, BtsCI bottom-strand cleavage site; black arrows, FokI cleavage sites.