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. 2009 Dec 3;38(4):1228–1239. doi: 10.1093/nar/gkp1102

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — DRAP1 occupancy at pol III-transcribed genes. HeLa cells were used for ChIP. (A) A polyclonal DRAP1 antibody (sc-17272) was used to test for DRAP1 presence at pol III-transcribed genes. TBP, Brf1 and pol III antibodies were used as positive controls. Goat IgGs and beads without antibody were used as negative controls. Input lanes show 10%, 2% and 0.4% of total input. (B) A monoclonal DRAP1 antibody (TM-301B-55) was used to confirm DRAP1 presence at pol III-transcribed genes, along with the Dr1 antibody 1162. TBP, Brf1 and pol III antibodies were used as positive controls; mouse IgGs and pre-immune (PI) serum were used as negative controls. Input lanes show 10%, 2% and 0.4% of total input.