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. 2009 Dec 6;38(4):1261–1272. doi: 10.1093/nar/gkp1113

Figure 6.

Figure 6.

Analysis of the cap-dependent and URE2 IRES-mediated translation in the wt mutant yeast strains. (A) Expression of reporter lacZ construct (13) under the control of the GAL1/10 promoter transformed into wt and mutant yeast strains. β-Galactosidase activity (relative units). (B) Expression of the monocistronic URE2 IRES p281-4-URE2 reporter lacZ construct (14,15) under the control of the GAL1/10 promoter transformed into wt and mutant yeast strains. β-Galactosidase activity was determined after a 20-h induction in 2% galactose at 30°C. Mean efficiencies determined from at least nine independent experiments are plotted and bars represent the corresponding standard error.