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. 2009 Dec 6;38(4):1261–1272. doi: 10.1093/nar/gkp1113

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Association of initiation factors eIF2 and eIF3 with 40S ribosomal subunits in wt and mutant (BY47yS5-30 and BY47yS5-46) yeast strains. Extracts from isogenic wt (BY47yS5-0) and rpS5 mutant (BY47yS5-30 and BY47yS5-46) strains were resolved by velocity sedimentation on 5–30% sucrose gradients as described (15). Western blot analyses of individual fractions were done using antibodies directed against the initiation factors eIF2α and eIF3b (PRT1) and the ribosomal protein S2 respectively. The positions of 40S subunits and 80S ribosomes are indicated. ‘In’ for input, represents a 7% portion of each gradient fraction.