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3E6 recognizes a conformational epitope on GFP. (A) The fluorescence of GFP, and (B) its recognition by 3E6, and a polyclonal anti-GFP antibody under different conditions, including (i) native, (ii) treated with SDS and (iii) treated with SDS and 100°C heat treatment. Bound GFP was detected using polyclonal anti-GFP and m3E6 antibodies followed by HRP labeled corresponding secondary antibodies.
