Figure 4.
(A) Epifluorescence image of a concentration profile in a membrane containing egg-PC, DOPS, and NBD-PE in an 85:14:1 mol ratio. This system is expected to behave like a two-component system because of the similarity in size and charge of NBD-PE and DOPS. A fluorescence intensity trace across the region is shown below with the location of the scratch boundaries marked with gray bars. A theoretical fit to the data (Eq. 18) is drawn with a solid line. A net force of 8.9 ± 0.4 × 10−17 N per molecule is predicted, which is consistent with the applied field strength of 10–15 V/cm. (B) Close-up view of the transition region of the profile illustrating its shape and extension of the calculated curve beyond the boundary.