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. Author manuscript; available in PMC: 2011 Jan 29.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jan 5;392(1):47–52. doi: 10.1016/j.bbrc.2009.12.158

Figure 4.

Figure 4

Leptin does not regulate tPA protein expression or activity. A) Representative Western blot and densitometry graph from four independent experiments showing absence of any changes in tPA expression with time after leptin (100 ng/ml) treatment. B) Representative Western blot and densitometry graph from four independent experiments showing no changes in tPA expression after treatment with increasing concentration of leptin. C) Graph from four independent experiments showing no changes in total tPA concentration in the conditioned media after treatment with increasing leptin concentrations. D) Graph from four independent experiments showing no changes in tPA activity in conditioned media from cells treated with increasing leptin concentrations. Data are presented as mean ±SEM.