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. Author manuscript; available in PMC: 2010 Mar 3.
Published in final edited form as: Mol Vis. 2000 Apr 5;6:24–29.

Figure 1.

Figure 1

Western immunoblotting of osteonectin/SPARC in lens extracts. Extracts were prepared and polypeptides were separated and blotted as described in Materials and Methods. Shown is the autoradiogram of the corresponding blot. Lane 1 contains purified osteonectin/SPARC, lane 2 contains cataractous lens extract (cat), and lane 3 contains normal lens extract (norm) probed with anti-SPARC antibody. Lane 4 contains cataractous lens extract (cat) and lane 5 contains normal lens extract (norm) probed with anti-αB-crystallin antibody. Also shown are the osteonectin/SPARC and the αB-crystallin bands with their corresponding molecular weights. The protein added to each well is shown below the blot.