Table 2.
Confirmation by RT-PCR
| Gene Symbol | Fold Change by Array | Fold Change by PCR | P Value by PCR |
|---|---|---|---|
| Sternohyoid > Diaphragm Expression | |||
| Pfkfb3 | 6.2 | 2.6 | 0.028 |
| Phka1 | 2.8 | 2.5 | < 0.001 |
| Abcc8 | 2.7 | 6.1 | < 0.001 |
| Ppp1cb | 2.7 | 1.5 | 0.035 |
| Phkg1 | 2.5 | 1.1 | 0.048 |
| Gpd2 | 2.4 | 4.1 | < 0.001 |
| Ppp1r2 | 2.2 | 1.3 | 0.037 |
| Diaphragm > Sternohyoid Expression | |||
| Pla2g2a | 37.9 | 13.5 | 0.002 |
| Harpb64 | 11.5 | 5.2 | < 0.001 |
| Fasn | 9.0 | 20.6 | 0.003 |
| Fabp3 | 5.3 | 8.0 | < 0.001 |
| Lipe | 5.0 | 9.0 | < 0.001 |
| Lpl | 4.8 | 8.6 | < 0.001 |
| Lsr | 4.6 | 37.7 | < 0.001 |
| Ncf1 | 4.4 | 16.2 | < 0.001 |
| Etfb | 4.1 | 4.5 | < 0.001 |
| Thrsp | 3.8 | 8.7 | 0.009 |
| Acaa2 | 3.7 | 6.5 | < 0.001 |
| Dci | 3.6 | 5.6 | < 0.001 |
| Peci | 3.2 | 3.5 | < 0.001 |
| Acadm | 2.8 | 1.9 | 0.003 |
| Apoe | 2.8 | 5.0 | < 0.001 |
| Cpt2 | 2.3 | 2.5 | < 0.001 |
| Nr0b2 | 2.3 | 10.8 | < 0.001 |
| Acadl | 2.2 | 3.5 | < 0.001 |
| St3gal5 | 2.2 | 2.5 | < 0.001 |
| Hadha | 2.2 | 3.9 | < 0.001 |
| Hadh2 | 2.1 | 4.7 | < 0.001 |
| Acaca | 2.1 | 2.8 | < 0.001 |
| Acly | 2.0 | 3.5 | 0.005 |
| Aldh3a2 | 2.0 | 2.1 | < 0.001 |
RT-PCR confirmation of differential lipid metabolism gene expression between sternohyoid and diaphragm muscles.